1.11.1.11 additional information - additional information of recombinant enzyme-B produced in E. coli 439881 1.11.1.11 additional information - additional information substrate: guaiacol, effects of Cys32Ser mutagenesis and DTNB-modification 439873 1.11.1.11 0.2 - ascorbate CCP2APX 696216 1.11.1.11 0.4 - 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, temperature not specified in the publication. Hill coefficient n is 1.0 726123 1.11.1.11 0.53 - ascorbate 25°C, wild-type enzyme 685400 1.11.1.11 1.5 - guaiacol pH 7.0, 25°C, mutant H42E 655435 1.11.1.11 1.74 - L-ascorbate 25°C 688913 1.11.1.11 1.83 - cytochrome c 25°C, wild-type enzyme 685400 1.11.1.11 2.03 - H2O2 25°C 688913 1.11.1.11 2.5 - guaiacol 25°C, wild-type enzyme 685400 1.11.1.11 3.9 - L-ascorbate pH 7.0, 25°C, mutant H42E 655435 1.11.1.11 27.24 - H2O2 pH 7.0, 25°C 764871 1.11.1.11 29.7 - H2O2 pH 7.0, temperature not specified in the publication 743788 1.11.1.11 40.42 - L-ascorbate pH 7.0, 25°C 764871 1.11.1.11 46.71 - H2O2 - 689240 1.11.1.11 49.6 - L-ascorbate pH 7.0, temperature not specified in the publication 743788 1.11.1.11 66 - guaiacol pH 7.0, 25°C, wild-type 655435 1.11.1.11 71.5 - L-ascorbate pH 7.4, 37°C 724680 1.11.1.11 84.5 - H2O2 pH 7.4, 37°C 724680 1.11.1.11 156 - L-ascorbate mutant S160M, pH 7.0, 25°C 655789 1.11.1.11 159 - L-ascorbate wild-type, pH 7.0, 25°C 655789 1.11.1.11 160 - pyrogallol wild-type, Cys32Ser mutant and DTNB-modified enzyme 439873 1.11.1.11 161 - L-ascorbate pH 7.0, temperature not specified in the publication. Non-Michaelis-Menten kinetics, Hill ccoefficient n is 1.6 726123 1.11.1.11 248 - L-ascorbate pH 7.0, 25°C, wild-type 655435 1.11.1.11 260 - L-ascorbate - 689240 1.11.1.11 577 - H2O2 recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C 711139 1.11.1.11 696 - ascorbate - 439871 1.11.1.11 754 - L-ascorbate recombinant full length enzyme, in 50 mM potassium phosphate buffer, pH 7.0, at 22°C 711139 1.11.1.11 760 - L-ascorbate in 50 mM potassium phosphate (pH 7.0), at 22°C 713299 1.11.1.11 1230 - H2O2 mutant enzyme C25S 686710 1.11.1.11 1310 - H2O2 mutant enzyme C25S/C121S 686710 1.11.1.11 1490 - L-ascorbate mutant enzyme C25S/C121S 686710 1.11.1.11 1570 - L-ascorbate mutant enzyme C25S 686710 1.11.1.11 1580 - H2O2 mutant enzyme C26S 684930, 686710 1.11.1.11 1630 - H2O2 mutant enzyme C126A 684930 1.11.1.11 1710 - L-ascorbate mutant enzyme C126A 684930 1.11.1.11 1730 - H2O2 mutant enzyme C26S/C126A 684930 1.11.1.11 1800 - H2O2 wild-type enzyme 684930, 686710 1.11.1.11 1910 - L-ascorbate mutant enzyme C26S/W35F/C126A 684930 1.11.1.11 1980 - L-ascorbate wild-type enzyme 686710 1.11.1.11 2040 - L-ascorbate mutant enzyme C26S 684930, 686710 1.11.1.11 2190 - H2O2 wild-type enzyme 686710 1.11.1.11 2400 - L-ascorbate mutant enzyme C26S/C126A 684930 1.11.1.11 2510 - L-ascorbate wild-type enzyme 684930, 686710 1.11.1.11 2833 - L-ascorbate mutant enzyme W35F 684930 1.11.1.11 3280 - H2O2 mutant enzyme C26S/W35F/C126A 684930 1.11.1.11 3410 - H2O2 mutant enzyme W35F 684930