1.1.1.50 additional information - additional information cofactor kinetics of wild-type and mutant enzymes, overview 684676 1.1.1.50 additional information - additional information overview, values for various substrates and 6 different isozymes 389526 1.1.1.50 0.000016 - 3alpha-hydroxytibolone below, pH 7.0, 37°, isozymes AKR1C1 and AKR1C3 656838 1.1.1.50 0.000016 - 3beta-hydroxytibolone below, pH 7.0, 37°, isozyme AKR1C3 656838 1.1.1.50 0.00017 - allopregnanolone pH 7.0, 25°C 654626 1.1.1.50 0.00017 - NADP+ pH 7.0, 25°C 654626 1.1.1.50 0.0005 - 3beta-hydroxytibolone pH 7.0, 37°, isozyme AKR1C1 656838 1.1.1.50 0.0018 - androsterone pH 7.4, 25°C, recombinant isozyme AKR1C4 656787 1.1.1.50 0.0023 - 4-pregnen-3alpha,20beta-diol pH 7.0, 25°C, isozyme AKR1C3 656200 1.1.1.50 0.0025 - 4-pregnen-3alpha,20beta-diol pH 7.0, 25°C, isozyme AKR1C1 656200 1.1.1.50 0.0028 - androsterone pH 9.0, 25°C, recombinant His-tagged mutant S114A/Y155F 669389 1.1.1.50 0.003 - 5alpha-dihydroprogesterone pH 7.0, 25°C 654626 1.1.1.50 0.003 - androsterone pH 9.0, 25°C, recombinant His-tagged mutant Y155F/K159A 669389 1.1.1.50 0.003 - NADPH pH 7.0, 25°C 654626 1.1.1.50 0.0035 - pregn-4-en-3,20-dione pH 7.4, 37°C, recombinant wild-type enzyme 738928 1.1.1.50 0.0038 - 5alpha-dihydrotestosterone pH 7.0, 37°C, with NAD(P)H 655348 1.1.1.50 0.004 - (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide pH 7.0, 37°C with NADP+ 655348 1.1.1.50 0.004 - 3beta-hydroxytibolone pH 7.0, 37°, isozyme AKR1C4 656838 1.1.1.50 0.004 - 5alpha-dihydroprogesterone isozyme AKR1C3 655790 1.1.1.50 0.007 - androsterone pH 7.4, 25°C, recombinant isozyme AKR1C2 656787 1.1.1.50 0.00783 - androsterone - 389547 1.1.1.50 0.008 - 3alpha-androstanediol pH 7.0, 25°C, recombinant enzyme 672088 1.1.1.50 0.008 - 5alpha-dihydroprogesterone pH 7.0, 25°C, isozyme AKR1C2 654924 1.1.1.50 0.012 - 3alpha-hydroxytibolone pH 7.0, 37°, isozyme AKR1C4 656838 1.1.1.50 0.015 - benzenedihydrodiol pH 7.4, 25°C, recombinant isozyme AKR1C3 656787 1.1.1.50 0.016 - 3beta-hydroxytibolone pH 7.0, 37°, isozyme AKR1C1 656838 1.1.1.50 0.0167 - (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide pH 7.0, 37°C with NAD+ 655348 1.1.1.50 0.0167 - 5beta-dihydroprogesterone pH 7.0, 37°C 654626 1.1.1.50 0.0167 - stanolone - 389540 1.1.1.50 0.018 - 5beta-dihydroprogesterone pH 7.0, 25°C, isozyme AKR1C2 654924 1.1.1.50 0.02 - 5alpha-dihydrotestosterone isozyme AKR1C2 655214 1.1.1.50 0.02 - benzenedihydrodiol pH 7.4, 25°C, recombinant isozyme AKR1C2 656787 1.1.1.50 0.02 - NADH mutant S114A, pH 7.5, 25°C 696459 1.1.1.50 0.022 - Dehydrolithocholic acid pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.03 - benzenedihydrodiol pH 7.4, 25°C, recombinant isozyme AKR1C4 656787 1.1.1.50 0.033 - 5alpha-dihydrotestosterone pH 7.0, 25°C, recombinant enzyme 672088 1.1.1.50 0.0333 - androsterone - 389540 1.1.1.50 0.0392 - pregn-4-en-3,20-dione pH 7.4, 37°C, recombinant mutant V54L 738928 1.1.1.50 0.0398 - 5alpha-dihydrotestosterone detection fluorometrically 389547 1.1.1.50 0.04 - 5alpha-dihydroprogesterone pH 7.0, 25°C, isozyme AKR1C1 654924 1.1.1.50 0.045 - cholate 37°C, pH 8.0, mutant enzyme K157A 760734 1.1.1.50 0.046 - 4-pregnen-3alpha,20beta-diol pH 7.0, 25°C, isozyme AKR1C2 656200 1.1.1.50 0.047 - 5beta-androstane-3,17-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.05 - 5beta-dihydroprogesterone pH 7.0, 25°C, isozyme AKR1C1 654924 1.1.1.50 0.05 - benzenedihydrodiol pH 7.4, 25°C, recombinant isozyme AKR1C1 656787 1.1.1.50 0.0612 - 5alpha-dihydrotestosterone pH 7.4, 37°C, recombinant wild-type enzyme 738928 1.1.1.50 0.0735 - 4-pregnen-3alpha,20beta-diol pH 7.0, 25°C, isozyme AKR1C4 656200 1.1.1.50 0.0757 - testosterone - 389547 1.1.1.50 0.0833 - 3alpha-adiol - 389540 1.1.1.50 0.09 - 1-Indanol pH 7.4, 25°C, recombinant isozyme AKR1C3 656787 1.1.1.50 0.0965 - 5alpha-dihydrotestosterone detection spectrophotometrically 389547 1.1.1.50 0.1 - 6-tert-butyl-2,3-epoxy-4-hydroxy-5-cyclohexen-1-one pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.105 - 5beta-pregnane-3,20-dione pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.114 - 5alpha-dihydrotestosterone detection radiometrically 389547 1.1.1.50 0.115 - (5beta,20R)-20-hydroxypregnan-3-one pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.1153 - 5alpha-dihydrotestosterone pH 7.4, 37°C, recombinant mutant V54L 738928 1.1.1.50 0.12 - androsterone pH 9.0, 25°C, recombinant His-tagged mutant S114A 669389 1.1.1.50 0.12 - cholate 37°C, pH 8.0, mutant enzyme S114A/Y153F 760734 1.1.1.50 0.13 - 1-Acenaphthenol pH 7.4, 25°C, recombinant isozyme AKR1C4 656787 1.1.1.50 0.133 - androsterone - 389540 1.1.1.50 0.14 - oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one isozyme AKR1C3 655790 1.1.1.50 0.158 - 1-Indanol pH 7.4, 25°C, recombinant isozyme AKR1C4 656787 1.1.1.50 0.163 - 1-Indanol pH 7.4, 25°C, recombinant isozyme AKR1C2 656787 1.1.1.50 0.167 - diacetyl pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.183 - 3alpha-adiol - 389540 1.1.1.50 0.183 - 9,10-phenanthrenequinone pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.203 - (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection spectrophotometrically 389547 1.1.1.50 0.21 - 1-Indanol pH 7.4, 25°C, recombinant isozyme AKR1C1 656787 1.1.1.50 0.283 - isatin pH 7.4, 25°C, recombinant wild-type AKR1C17 684676 1.1.1.50 0.32 - (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection radiometrically 389547 1.1.1.50 0.42 - 1-Acenaphthenol pH 7.4, 25°C, recombinant isozyme AKR1C3 656787 1.1.1.50 0.42 - 5alpha-androstane-3,17-dione pH 7.0, 25, reduction reaction 687635 1.1.1.50 0.444 2.6 androsterone - 389526 1.1.1.50 0.49 - 1-Acenaphthenol pH 7.4, 25°C, recombinant isozyme AKR1C1 656787 1.1.1.50 0.507 - (2Z)-2-(5-hydroxy-4,6-dimethyl-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N,N-di(prop-2-en-1-yl)hydrazinecarbothioamide detection fluorometrically 389547 1.1.1.50 0.59 - 1-Acenaphthenol pH 7.4, 25°C, recombinant isozyme AKR1C2 656787 1.1.1.50 0.62 - cholate 37°C, pH 8.0, mutant enzyme Y153F 760734 1.1.1.50 0.8 - cholate 37°C, pH 8.0, mutant enzyme S114A 760734 1.1.1.50 0.82 - androsterone pH 7.0, 25, oxidation reaction 687635 1.1.1.50 0.89 - dehydrocholate 37°C, pH 8.0, mutant enzyme S114A 760734 1.1.1.50 1 - 5alpha-dihydrotestosterone isozyme AKR1C9 655214 1.1.1.50 1.08 - 4-nitrobenzaldehyde - 389547 1.1.1.50 1.1 - dehydrocholate 37°C, pH 8.0, mutant enzyme Y153F 760734 1.1.1.50 1.5 - androsterone pH 9.0, 25°C, recombinant His-tagged mutant Y155F 669389 1.1.1.50 1.9 - androstanedione detection spectrophotometrically 389547 1.1.1.50 2.2 - 2-decalol pH 10.5, 25°C 760866 1.1.1.50 2.5 - Cyclohexanol pH 10.5, 25°C 760866 1.1.1.50 18.9 - 9,10-phenanthrenequinone - 389547 1.1.1.50 18.9 - androsterone pH 9.0, 25°C, recombinant His-tagged mutant K159A 669389 1.1.1.50 23 - cholate 37°C, pH 8.0, wild-type enzyme 760734 1.1.1.50 23 - Fusidic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 25 - Fusidic acid pH 8.5, 37°C, with cofactor thio-NAD+ 656300 1.1.1.50 35 - cholic acid pH 8.5, 37°C, with cofactor thio-NAD+ 656300 1.1.1.50 48 - dehydrocholate 37°C, pH 8.0, wild-type enzyme 760734 1.1.1.50 63 - NADH wild-type, pH 7.5, 25°C 696459 1.1.1.50 72 - lithocholic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 74 - taurocholic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 75 - cholic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 76 - chenodeoxycholic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 77 - glycocholic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 82 - (3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 134 - androsterone pH 8.5, 37°C, with cofactor NAD+ 656300 1.1.1.50 230 - androstenol pH 10.5, 25°C 760866 1.1.1.50 318 - androsterone pH 9.0, 25°C, recombinant His-tagged wild-type 669389 1.1.1.50 500 - androsterone pH 10.5, 25°C 760866