6.3.5.3 42 80 the first unfolding transition at 42°C leads to a loss of 15-20% of the secondary structure content of the protein and 80% loss in activity. The second unfolding transition at 80°C represents unfolding of the bulk of the protein 728631 6.3.5.3 50 - 5 min, up to 98% loss of activity. A combination of Gln, MgCl2, and KCl protects against thermal inactivation 1669