4.2.1.53 20 70 after 1 h incubation, the enzyme remains stable between 20 and 40°C (100% activity), at 45°C, 50°C, 55°C, 60°C, and 70°C it shows 80%, 70%, 45%, 20% and 0% residual activity, respectively 729071 4.2.1.53 25 35 after 120 min, the enzyme activity is nearly unchanges at 25°C, but is reduced to only 25% at 35 °C 729334 4.2.1.53 50 - the immobilized enzyme (covalent immobilization of the enzyme using glutaraldehyde as cross-linking agent with chitosan-magnetic composite as carrier) preserves 40% of the initial activity, while the native enzyme is completely inactivated 748410