2.4.1.18 additional information - high thermal tolerance even at boiling temperatures 721012 2.4.1.18 additional information - the activity is lost after incubation at 50°C for 30 min 674221 2.4.1.18 additional information - when the enzymes are maintained for 15 min at various temperatures ranging from 30°C to 60°C, more than 80% of the original activity was retained up to 47.5°C for PvSBE2, up to 40°C for D15A- and D15E-PvSBE2, up to 42.5°C for R28A- and H24A-PvSBE2, and up to 45°C for R28K-PvSBE2. 676385 2.4.1.18 - 45 the enzyme retains about 80% of its activity at 0-45° for 1 h 756764 2.4.1.18 5 45 no apparent inactivation up to 30°C, about 10% loss of activity at 35°C, about 90% loss of enzyme activity at 50°C 705081 2.4.1.18 10 30 pre-incubation of the enzyme for 2 h at different temperatures, following enzyme assay is conducted at 30°C. About 80% activity is retained after pre-incubation at 37°C and 45°C 676973 2.4.1.18 10 40 purified recombinant enzyme, the enzyme retains about 80% of its activity at 40°C for 30 min, the enzyme displays excellent cold adaptation over a low temperature range of 10-30°C, retaining over 85% of its relative activity within this range 736135 2.4.1.18 35 - pH 8.0, 30 min, stable below 636932 2.4.1.18 35 - purified recombinant His-tagged isozyme BE3, stable up to 735716 2.4.1.18 35 - the half-life of the enzyme at 35°C is 10 h 756961