1.5.8.4 47 - melting point of mutant enzyme H109R 742499 1.5.8.4 47 - melting point, mutant H109R 742499 1.5.8.4 52 - melting point of wild-type enzyme 742499 1.5.8.4 52 - melting point, wild-type 742499 1.5.8.4 52 - melting temperature 742499