3.5.5.5 15 55 exposure of the enzyme at 15, 25, and 35°C for 5 h causes 8%, 15%, and 28% decrease in arylacetonitrilase activity, respectively. A further increase in the temperature to 45 and 55°C results in 58% and 90% decrease in activity, respectively. The half-life of this enzyme at 15, 25, 35, 45, 50, and 55°C is 31 h, 16 h, 9 h, 4 h 30 min, 3 h 20 min, and 2 h 40 min, respectively 733546 3.5.5.5 20 - 40 h, about 50% loss of activity 756265 3.5.5.5 25 - purified recombinant enzyme, pH 8.0, 1 h, 90% activity remaining 718670 3.5.5.5 25 35 purified recombinant enzyme, pH 8.0, 1 h, stable 718670 3.5.5.5 30 - 10 h, about 50% loss of activity 756265 3.5.5.5 30 - 6 h, 87% residual activity 667345 3.5.5.5 30 - and below, 30 min, stable in 10 mM potassium phosphate buffer, pH 7.5 32503 3.5.5.5 35 - 30 min, in 10 mM potassium phosphate buffer, pH 7.5, 25% loss of activity 32503 3.5.5.5 35 - purified recombinant enzyme, pH 8.0, 1 h, 80% activity remaining 718670 3.5.5.5 35 65 purified recombinant enzyme, labile at higher temperature, half-life of 57 min at 40°C, 42 min at 45°C, and 20 min at 55°C, respectively. The activity decreases markedly above 55°C 719629