3.5.4.5 50 - relatively heat stable, no loss of activity when heated for 15 min, activity completely lost after heating at 75°C 209649 3.5.4.5 60 - relatively labile to thermal treatment, complete inactivation by incubation for 30 min 209660 3.5.4.5 50 - 15 min, absence of dithiothreitol, 44% residual activity 650635 3.5.4.5 100 - 1 h, 1 mM dithiothreitol, 48% residual activity 650635 3.5.4.5 100 - 1 h, presence of D,L-lipoic acid, pH 7.0, 75% residual activity 650635 3.5.4.5 100 - 15 min, 1 mM dithiothreitol, 80% residual activity 650635 3.5.4.5 100 - 2 h, 50 mM dithiothreitol, 56% residual activity 650635 3.5.4.5 65 - stable up to 657220 3.5.4.5 72 - 30 min, irreversible denaturation 657220 3.5.4.5 80 - stable for at least 60 min 669137