2.3.1.101	additional information	-	at cyclic 2,3-diphosphoglycerate concentrations prevailing in the cells of Methanopyrus kandleri the enzyme is completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cyclic 2,3-diphosphoglycerate confer thermostability to the enzymes	726828	
2.3.1.101	additional information	-	the enzyme is thermostable	661043	
2.3.1.101	65	-	stable for more than 4 h	486089	
2.3.1.101	70	-	up to, in presence of 1.5 M K2HPO4, hyperthermophilic enzyme	486092	
2.3.1.101	80	-	50% inactivation after 20 min at 0.005 M K3PO4. No inactivation after 20 min at 0.05 M K3PO4	726443	
2.3.1.101	80	-	50% inactivation after 20 min at 0.05 M K3PO4. No inactivation after 20 min at 0.5 M K3PO4	726443	
2.3.1.101	80	-	50% inactivation after 20 min at 1.2 M K3PO4. No inactivation after 20 min at 1.5 M K3PO4	726443	
2.3.1.101	80	-	complete thermostability in presence of 0.5 M K2HPO4	486086	
2.3.1.101	90	-	30 min, wild-type enzyme, loss of 70% activity at 0.8-1.0 M potassium phosphate	661043	
2.3.1.101	90	-	in 0.5 M 2,3-diphosphoglycerate, the cyclohydrolase is completely stable at pH 8.0 and 90°C.	726828