1.5.5.2 additional information - enzyme unfolding experiments using recombinant MBP-tagged enzyme in 20 mM Hepes, pH 8.0, at 60-95°C, the MBP-tagged enzyme unfolds at lower temperature around 56°C due to unfolding of the MBP tag 742162 1.5.5.2 45 - 50% loss of activity of wild-type PutA86-669 after 1 h, 50% loss of of L432P mutant form of PutA86-669 after 8 min 658033 1.5.5.2 70 - 10 min, about 90% of the activity remains 658668 1.5.5.2 70 - 10 min, fully active 392570 1.5.5.2 70 - the alpha4beta4-type PDHbeta retains more than 80% of its activity after heating at 70°C for 20 min 741732 1.5.5.2 70 - the alphabetagammadelta-type PDHbeta nearly loses all of its activity during incubation at 70°C for 20 min 741732 1.5.5.2 70 - the alphabetagammadelta-type PDHbeta retains 100% of activity during incubation at 70°C for 20 min 741732 1.5.5.2 70 - the alphabetagammadelta-type PDHbeta retains 68% of activity during incubation at 70°C for 20 min 741732 1.5.5.2 70 - the alphabetagammadelta-type PDHbeta retains 76% of activity during incubation at 70°C for 20 min 741732 1.5.5.2 80 - 120 min, 40% of activity remains 392570