1.14.99.53	additional information	-	chitin binding increases the thermal stability of the enzyme by 8.3 degrees, copper binding increases melting temperature by 21.6 degrees. Addition of chitin to the copper-bound enzyme increases thermal stability by additional 3.5 degrees	740345	
1.14.99.53	additional information	-	thermal unfolding of both apo- and holoCBP21 is reversible. ApoCBP21 unfolds in a simple two-state transition manner. The peak temperature of the differential scanning calorimetry curve, tp, for holoCBP21is (74.4°C and that for apoCBP21 is 65.6°C). HoloCBP21 is stabilized by 35 kJ/mol in terms of the Gibbs energy change for unfolding (pH 5.0, 25°C), compared with apoCBP21	766321	
1.14.99.53	49.3	-	melting temperature, presence of EDTA	766292	
1.14.99.53	59.2	-	melting temperature	766292	
1.14.99.53	64.1	-	wild-type, melting point	767106	
1.14.99.53	68.7	-	melting temperature, presence of both copper and chitin	740345