1.10.3.3 additional information - - 439924 1.10.3.3 additional information - different molecular forms vary in resistance to heat inactivation: tetramer of squash and dimer of cucumber being most resistant 439924 1.10.3.3 additional information - enzyme thermal stability in freshly harvested 4, 6, or 8 weeks old leaves and in leaves of the same ages stored at -80°C and thawed at 25°C, overview. In situ thermal inactivation of AAO in cowpea leaves follows first-order kinetics with an Arrhenius dependence 725176 1.10.3.3 additional information - role of copper in heat stability 439912 1.10.3.3 additional information - the thermal inactivation is irreversible, a first-order reaction mechanism 672768 1.10.3.3 - 40 30 min, stable 439920 1.10.3.3 10 - half-life: 1047 min 672768 1.10.3.3 12 - stable for at least 30 days 439919 1.10.3.3 15 - immobilized enzyme retains full activity for 3 months at pH 5.0-7.0, free enzyme looses 40-70% activity within one day at pH 5.0-7.0 439911 1.10.3.3 40 - 24 h, the enzyme retains 70% of its ascorbate oxidase activity 764908