1.1.1.71	additional information	-	thermal denaturation is fully irreversible and follows first-order kinetics	726699	
1.1.1.71	4	60	the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C	737536	
1.1.1.71	8	25	the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)	740200	
1.1.1.71	30	-	no loss of activity after 15 min, complete loss of activity at 60°C	287280	
1.1.1.71	41	-	melting temperature T1/2 of wild-type enzyme ADHA	760769	
1.1.1.71	48	-	melting temperature T1/2 of mutant ADHA-0278	760769	
1.1.1.71	53	-	melting temperature T1/2 of mutant ADHA-0279	760769	
1.1.1.71	65	-	melting temperature T1/2 of mutant ADHA-0391	760769	
1.1.1.71	66	-	melting temperature T1/2 of mutant ADHA-0384	760769	
1.1.1.71	67	-	melting temperature T1/2 of mutant ADHA-0398 and of mutant ADHA-0381	760769