3.5.1.13 37 40 purified recombinant enzyme, pH 9.0, the half-life of enzyme activity at 37°C is 192 h, and 90% of its activity remain after 3 h incubation at 40°C 713035 3.5.1.13 40 - 9.5 min, 50% loss of activity 208988 3.5.1.13 40 - fairly stable up to 40°C, completely inactivated during 30 min at 60°C 718672 3.5.1.13 40 - purified His6-tagged enzyme, pH 8.0, stable at for 1 h 733190 3.5.1.13 40 - purified recombinant enzyme, fairly stable up to, over 90% activity remaining after 1 h 718672 3.5.1.13 40 60 the purified recombinant wild-type enzyme is fairly stable up to 40°C, has 25% residual activity at 50°C after 1 h, and is completely inactivated at 60°C after 30 min 718672 3.5.1.13 45 - 2 min, 50% loss of activity 208988 3.5.1.13 45 - 5 min, enzyme retains high activity 208985 3.5.1.13 45 - 5 min, stable up to 208986 3.5.1.13 45 - purified recombinant enzyme, 3 h, pH 9.0, stable up to 713035 3.5.1.13 45 50 purified His6-tagged enzyme, pH 8.0, 1 h, 50% activity remaining 733190 3.5.1.13 46 48 5 min, 50% loss of activity 208985 3.5.1.13 50 - 0.5 min, 50% loss of activity 208988 3.5.1.13 50 - 25% residual activity after 1 h at 50°C, completely inactivated during 30 min at 60°C 718672 3.5.1.13 50 - half life time: 35 min 696876 3.5.1.13 50 - purified recombinant enzyme, 25% activity remaining after 1 h 718672 3.5.1.13 50 - stable up to 208980 3.5.1.13 55 - purified recombinant enzyme, 3 h, pH 9.0, 50% activity remaining 713035 3.5.1.13 60 - 20 min, complete inactivation 208973 3.5.1.13 60 - purified His6-tagged enzyme, pH 8.0, 1 h, inactivation 733190 3.5.1.13 60 - purified recombinant enzyme, 30 min, complete inactivation 718672 3.5.1.13 60 - stable for at least 90 min 208980 3.5.1.13 68 - 5 min, complete inactivation 208985 3.5.1.13 70 - 10 min, complete inactivation 208980 3.5.1.13 70 - purified recombinant enzyme, 3 h, pH 9.0, inactivation 713035