3.2.1.157 - 50 over 80% activity remaining at 0-30°C, 5-10% at 50°C 753830 3.2.1.157 30 - purified recombinant His-tagged enzyme, stable up to, 97% and 84% of the initial activity is retained after 24 h at 4°C and 25°C 754224 3.2.1.157 40 - purified recombinant enzyme, stable up to, inactivation at 50°C 755283 3.2.1.157 40 - purified recombinant His-tagged enzyme, pH 6.5, 1 h, 80% activity remaining 731522 3.2.1.157 40 - purified recombinant His-tagged enzyme, pH 6.5, 1 h, inactivation 731522 3.2.1.157 40 - stable up to 731522 3.2.1.157 45 - the enzyme is stable up to 45°C in 50 mM Tris/HCl, pH 7.5, containing 0.1 M NaCl and 1 mM CaCl2 for 1 h. Incubation at 45°C inactivates the enzyme completely within 5 min at either condition of low NaCl concentration (less than 10 mM) or presence of 10 mM EDTA 716089 3.2.1.157 50 - purified enzyme, 1 h, over 80% activity remaining 755018 3.2.1.157 50 - purified recombinant His-tagged enzyme, pH 6.5, 1 h, 60% activity remaining 731522