2.7.7.43 22 - not stable at room temperature for 12 h 645255 2.7.7.43 25 - enzyme is more stable at lower temperatures, 55% of enzyme activity remains after 24 h incubation at 25°C 672738 2.7.7.43 25 - unstable even at 645268 2.7.7.43 35 - 60 min, completely stable 645261 2.7.7.43 37 - 60 min, about 10% loss of activity 645261 2.7.7.43 37 - enzyme is not stable at 37°C, incubation for 1 h decreases enzyme activity by 50%, incubation for 24 h at 37°C completely inactivates enzyme activity 672738 2.7.7.43 37 - reaction rate is proportional to enzyme concentration and constant for at least 45 min. At high enzyme concentrations the reaction rate decreases 10-15% between 45 and 60 min 645255 2.7.7.43 37 70 complete loss of activity above 70°C, no loss of activity after 24 h incubation at 37°C and 50°C 671567 2.7.7.43 40 - 50 min, complete inactivation without stabilizer, completely stable in presence of 1.0 M NaCl, 0.5 M Na2HPO4 or 25% v/v glycerol 645270 2.7.7.43 40 - 60 min, 20% loss of activity 645261 2.7.7.43 40 45 the enzyme loses complete activity after 50 and 10 min when stored at 40°C and 45°C, respectively 690583 2.7.7.43 45 - 10 min, complete deactivation 645270 2.7.7.43 45 - 60 min, 65% loss of activity, less than 10% loss of activity in presence of 20% w/v glycerol 645261 2.7.7.43 50 - 10 min, 85% loss of activity 645261 2.7.7.43 55 - 20 min, complete loss of activity 645261 2.7.7.43 70 - the enzyme retains activity at 70°C 690583