2.5.1.56	additional information	-	the enzyme from a psychrophilic bacterium, MvNeuB, is less stable at higher temperature and has a high catalytic activity at lower temperature compared to mesophilic counterparts. MvNeuB is a typical cold adapted enzyme	737983	
2.5.1.56	additional information	-	the wild-type apoenzyme has a higher melting temperature of 42.6°C compared to the mutant G272Term apoenzyme with 36.5°C. Unlike the wild-type enzyme, the melting temperature of mutant G272Term in the presence of Mn2+ remains consistent and is not influenced by the addition of different ligand combinations. The melting temperature of wild-type NmeNANAS increases slightly in the presence of Mn2+	739614	
2.5.1.56	30	-	pH 7.5, 30 min, stable up to	637321	
2.5.1.56	37	-	thermostable up to	637325	
2.5.1.56	40	-	pH 7.5, 30 min, about 60% loss of activity	637321	
2.5.1.56	40	60	purified recombinant enzyme, pH 8.0, reasonably stable, maintaining 60% activity after 4 h at 40, and 50% activity after 30 min at 50°C, and almost fully inactivated at 60°C after 1 h	738339	
2.5.1.56	50	-	30 min, 80-90% loss of activity	637325	
2.5.1.56	50	-	pH 7.5, 30 min, about 80% loss of activity	637321	
2.5.1.56	60	-	pH 7.5, 30 min, complete loss of activity	637321