2.4.1.9 additional information - Ca2+ stabilizes the enzyme 658750 2.4.1.9 35 - the wild type enzyme shows a half-life of 2.2 h at 35°C 702002 2.4.1.9 35 - wild-type, half-life 420 min, truncated versions IslA2, half-life 407 min, IslA3, 346 min, IslA4, 128 min 685806 2.4.1.9 35 50 the enzyme shows 50% activity after 420 min at 35°C and is inactive after 30 min at 50°C 756236 2.4.1.9 40 - half-life of wild-type enzyme: 70 min, half-life of the two C-terminally truncated mutant enzymes is 20 min and 12 min 489489 2.4.1.9 40 - the enzyme is stable up to 40°C 702814 2.4.1.9 50 - the enzyme shows 84% activity after 180 min at 50°C 756236 2.4.1.9 50 - the immobilized enzyme retains about 84% of activity after incubation at 50°C for 12 h, whereas the residual activity of the free enzyme is lower than 50% under the same conditions 758340 2.4.1.9 50 60 the enzyme maintains 84% activity after incubation at 50°C for 3 h. 44% of the residual activity remains after 1 h at 55°C, and the enzyme loses 50% of its initial activity after incubation at 60°C for 20 min 756939