1.4.1.13 -20 - complete loss of activity after 10 days 391455 1.4.1.13 -10 - stable for at least 6 weeks 391452 1.4.1.13 - - stable for 6-8 h in crude extract 391458 1.4.1.13 4 - unprotected enzyme loses 90% of activity in 48 hours, protected enzyme, addition of 5 mM EDTA and 5 mM 2-mercaptoethanol or 1 mM DTT retains 80 to 90% of the activity after 48 hours 391455 1.4.1.13 40 - 10 min, 80% loss of activity, enzyme in crude extract 391458 1.4.1.13 45 - 3 min, 80% loss of activity, enzyme in crude extract 391458 1.4.1.13 45 - the enzyme retains its full activity after heating for 10 min in 0.1 M potassium phosphate buffer, pH 6.8 391448 1.4.1.13 55 - 10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity 391448 1.4.1.13 70 100 purified enzyme, 10 min, stable up to 90°C 725886 1.4.1.13 80 - 10 min, 35% loss of activity 391451 1.4.1.13 80 - enzyme extract is clearly very stable 745714 1.4.1.13 100 - 30 s, complete loss of activity 391455 1.4.1.13 100 - purified enzyme, 10 min, loss of activity 725886