1.2.1.4 7 9.5 Tm of wild-type enzyme 1.2.1.4 50 - stable up to 1.2.1.4 60 - loss of activity above 1.2.1.4 72.5 - Tm of mutant D176S/S206K/M262I/W271Y/W275V 1.2.1.4 78.5 - Tm of mutant D176S/S206K/M262I