1.2.1.22	40	55	purified recombinant wild-type enzyme is stable at 40°C after incubation for 1 h, but its stability decreases rapidly above 40°C. The mutants N286E and N286H show the same stability, decreasing rapidly above 40°C and exhibiting 20% and 40% of their maximum activity at 45°C, respectively. In contrast, mutant N286T is stable above 50°C and retains 60% of its maximum activity at 55°C after incubation for 1 h	
1.2.1.22	50	-	pH 6.5, 10 min, 50% loss of activity	
1.2.1.22	55	-	1 h, 70% residual activity for mutant N286T	
1.2.1.22	80	-	86% activity after 80°C for 10 min	
1.2.1.22	90	-	45% activity after 90°C for 10 min	
1.2.1.22	100	-	25% activity after 100°C for 10 min