1.12.2.1 4 - 4 weeks, atmosphere of N2, air or H2, very little loss of activity 395560 1.12.2.1 23 - atmosphere of N2, air or H2, 40% loss of activity after 2 weeks, complete loss of activity after 4 weeks 395560 1.12.2.1 35 40 at optimal temperature range of 35-40°C, a minimal activity loss during the first 10 to 20 min and thereafter, activity steadily decreases as incubation time increases. After 80 min of incubation, the enzyme loses 75-80% of all of its activity 701586 1.12.2.1 50 - 10 min, H2 evolution in presence of methyl viologen, no decrease in activity 395561 1.12.2.1 55 - completely denatured above 701586 1.12.2.1 60 - 10 min, H2 evolution in presence of methyl viologen, 30% loss of activity 395561 1.12.2.1 70 - 10 min, H2 evolution in presence of methyl viologen, 37% loss of activity 395561 1.12.2.1 77 - stable below, 2 h 395560 1.12.2.1 80 - 10 min, H2 evolution in presence of methyl viologen, 69% loss of activity 395561 1.12.2.1 80 - stable below 395569 1.12.2.1 90 - 10 min, H2 evolution in presence of methyl viologen, 99% loss of activity 395561 1.12.2.1 100 - 10 min, H2 evolution in presence of methyl viologen, complete loss of activity 395561