1.1.1.71	additional information	-	thermal denaturation is fully irreversible and follows first-order kinetics	726699	
1.1.1.71	4	60	the enzyme is thermally stable when incubated for 30 min between 4 and 40°C. Incubation for 30 min at temperatures between 10 and 30 °C slightly increases the catalytic activity. Recombinant enzyme is completely inactivated at 50 and 60°C	737536	
1.1.1.71	8	25	the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)	740200	
1.1.1.71	30	-	no loss of activity after 15 min, complete loss of activity at 60°C	287280	
1.1.1.71	41	-	melting temperature T1/2 of wild-type enzyme ADHA	760769	
1.1.1.71	48	-	melting temperature T1/2 of mutant ADHA-0278	760769	
1.1.1.71	53	-	melting temperature T1/2 of mutant ADHA-0279	760769	
1.1.1.71	65	-	melting temperature T1/2 of mutant ADHA-0391	760769	
1.1.1.71	66	-	melting temperature T1/2 of mutant ADHA-0384	760769	
1.1.1.71	67	-	melting temperature T1/2 of mutant ADHA-0398 and of mutant ADHA-0381	760769	
1.1.1.71	70	-	1.5 h, no loss of activity	726712	
1.1.1.71	70	-	2 h, 10% loss of activity	726712	
1.1.1.71	70	95	the enzyme activity is slightly decreased by 1 h incubation at 70°C (2.6%), 80°C (4.7%), 90°C (7.9%), and 95°C (18.8%), 50% loss of activity after approximately 6 h at 95°C	761529	
1.1.1.71	80	-	1.5 h, no loss of activity	726712	
1.1.1.71	80	-	2 h, 15% loss of activity	726712	
1.1.1.71	80	-	activity begins to decrease after 2 h preincubation. The rate of 2-propanol oxidation is decreased 34% and 58% over 24 h incubation at 80°C in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 37 vs. 20 h at 80°C, respectively	726699	
1.1.1.71	90	-	activity begins to decrease after 30 min preincubation. The incubation at 90°C provokes 31% and 40% reduction of 2-propanol oxidation rate in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 8 vs. 6 h at 90 °C, respectively	726699	
1.1.1.71	90	-	half-life 2 h	726712	
1.1.1.71	90	-	half-life: 2 h	726712	
1.1.1.71	95	-	the highly thermostable enzyme has a half-life of 4.5 h at 95°C and 16.2 h at 85°C	735486	
1.1.1.71	100	-	half-life 1 h	726712	
1.1.1.71	100	-	half-life: 1 h	726712	
1.1.1.71	102	-	Tm-value in presence of 1 M NaCl	726699	
1.1.1.71	104	-	Tm-value in absence of NaCl	726699