3.1.1.41 50 - purified recombinant enzyme, pH 7.0, loss of 20% activity after 120 h 729073 3.1.1.41 50 - purified recombinant enzyme, pH 7.0, loss of 60% activity after 120 h 729073 3.1.1.41 60 - pH 7.0, 30 min, stable up to 170673 3.1.1.41 60 - purified recombinant enzyme, pH 7.0, inactivation within 10 h 729073 3.1.1.41 65 - pH 7.0, 30 min, 70% loss of activity 170673 3.1.1.41 70 - 10 min, loss of activity 170669 3.1.1.41 70 - pH 7.0, 30 min, complete loss of activity 170673 3.1.1.41 70 - salt-free enzyme, 5 min, 50% loss of activity 170666 3.1.1.41 80 - complete loss of activity after 5 min, activity is retained after 10 min in presence of 1.0 M phosphate buffer 170667 3.1.1.41 105 - the unfolding transition for the wild type occurs at a temperature higher than 105°C 749261