5.4.99.5	additional information	-	The average structures of the thermophilic E.S complex at three different temperatures are obtained from the MD simulations. A snapshot of the most important features of the active site of the TtCM E.S complex at 70°C is shown. At this temperature the Boltzmann distribution is primarily composed of reactive conformers - near attack conformers (NACs). The effects of the different temperatures on the distances in the structure are shown. The distances between electrostatic and hydrophobic pairs decrease as temperature decreases	672198	
5.4.99.5	20	-	assay at	671111	
5.4.99.5	35	37	enzyme form CM1	3564	
5.4.99.5	37	-	-	3536	
5.4.99.5	37	-	assay at	672268, 674346, 747259, 747842, 749355	
5.4.99.5	37	-	at assay	674312	
5.4.99.5	38	-	decrease in turnover at temperatures higher than 38°C	651660	
5.4.99.5	48	-	maximum enzymatic activity	651660	
5.4.99.5	56	-	enzyme form CM2	3564	
5.4.99.5	60	-	assay at	692260