5.4.99.16 15 - - 746863 5.4.99.16 15 - wild-type 680304 5.4.99.16 20 - - 3490 5.4.99.16 20 - assay at 746621, 747301 5.4.99.16 20 - wild-type enzyme and recombinant fusion enzyme mutant 747301 5.4.99.16 25 - - 746863 5.4.99.16 25 - recombinant His6-tagged enzyme 748492 5.4.99.16 30 - - 726558, 746863 5.4.99.16 30 - assay at 727655, 747324 5.4.99.16 30 - recombinant fusion enzyme mutant 747301 5.4.99.16 35 - - 681153, 701844, 715078, 746863, 759128, 760081 5.4.99.16 37 - - 705534, 746863 5.4.99.16 37 - assay at 721854, 727887 5.4.99.16 40 - - 718530, 746863 5.4.99.16 40 - both deletion mutant lacking 415 amino acids from C-terminus, and fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus 680304 5.4.99.16 40 - fusion protein of Deinococcus radiodurans enzyme N-terminus plus Thermus thermophilus enzyme C-terminus 680304 5.4.99.16 40 - immobilized enzyme 716805 5.4.99.16 40 - in 100 mM phosphate buffer 690584 5.4.99.16 40 - maximal yield of trehalulose from sucrose 3491 5.4.99.16 40 - recombinant enzyme 759622 5.4.99.16 40 - wild-type enzyme 747301 5.4.99.16 45 - - 3493, 680282, 728630, 746863 5.4.99.16 45 - free enzyme 716805 5.4.99.16 45 - recombinant enzyme 748015 5.4.99.16 50 - - 703606, 746863, 759128 5.4.99.16 50 - assay at 747681 5.4.99.16 50 - free and immobilized recombinant enzyme 748725 5.4.99.16 50 - free recombinant enzyme 746777 5.4.99.16 50 - recombinant enzyme 747518 5.4.99.16 50 - recombinant surface-displayed TreS 748005 5.4.99.16 50 - recombinant wild-type enzyme and mutant TSMrTt 727433 5.4.99.16 53 - free enzyme 728159 5.4.99.16 55 - recombinant enzyme 747445 5.4.99.16 60 - highest activity (76.8%) 759527 5.4.99.16 60 - immobilized recombinant enzyme 746777 5.4.99.16 60 - recombinant wild-type enzyme and mutant TSTtMr 727433 5.4.99.16 65 - - 3489, 746863, 749423 5.4.99.16 65 - wild-type 680304 5.4.99.16 70 - spherical aggregated enzyme 728159 5.4.99.16 70 - temperature optimum for enzyme and for recombinant fusion protein with N-terminal beta-amylase of Clostridium thermofluorogenes and C-terminal trehalose synthase or vice versa 680294 5.4.99.16 70 80 both free and chitosan-immobilized enzymes 705096