3.2.1.23 additional information - enzyme highly active at low temperatures: 60% at 0°C 714850 3.2.1.23 - 20 assay at, cold-active enzyme 679711 3.2.1.23 10 - - 682671, 701854 3.2.1.23 15 - - 655860, 715886 3.2.1.23 22 - assay at 678430 3.2.1.23 22 - assay at room temperature 727060 3.2.1.23 25 - - 701953 3.2.1.23 25 - assay at 680416, 682849 3.2.1.23 25 - thioglycoligase assay at 679834 3.2.1.23 26 - hydrolysis of o-nitrophenyl beta-D-galactopyranoside 654291 3.2.1.23 28 - - 715223 3.2.1.23 30 - - 171309, 657240, 701854 3.2.1.23 30 - assay at 678476, 681512 3.2.1.23 30 - glycohydrolase assay at 679834 3.2.1.23 30 - hydrolysis of o-nitrophenyl-beta-D-galactoside 171310 3.2.1.23 30 35 - 171190 3.2.1.23 30 40 - 701806 3.2.1.23 31 - assay at 677797 3.2.1.23 32 37 - 171318 3.2.1.23 35 - - 171190, 657569 3.2.1.23 35 - two optima, 35°C and 55°C 679236 3.2.1.23 35 - wild-type enzyme 681772 3.2.1.23 35 50 - 706705 3.2.1.23 37 - - 677630, 701854, 715886 3.2.1.23 37 - assay at 665269, 677571, 678007, 679564, 680208 3.2.1.23 37 - hydrolysis of o-nitrophenyl beta-D-galactopyranoside 655098 3.2.1.23 37 - in vivo fermentation temperature and assay at 680415 3.2.1.23 37 - pH 6.6 695674 3.2.1.23 37 39 hydrolysis of lactose or p-nitrophenyl-beta-D-galactoside 171352 3.2.1.23 37 40 - 701854 3.2.1.23 38 - - 713883 3.2.1.23 40 - - 171349, 665014, 666823, 700664, 701854, 744825 3.2.1.23 40 - assay at 678477 3.2.1.23 40 - hydrolysis of lactose 655098 3.2.1.23 40 - recombinant mutant hybrid enzyme 681772 3.2.1.23 42 - - 171302, 654248, 663740 3.2.1.23 42 - cell wall enzyme 171308 3.2.1.23 45 - - 171304, 171324, 171340, 655644, 663632, 714567 3.2.1.23 45 - hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside, strain L461 665287 3.2.1.23 45 - hydrolysis of lactose, strain L103 665287 3.2.1.23 45 - hydrolysis of o-nitrophenyl-beta-D-galactoside 171299 3.2.1.23 45 - native enzyme, substrate 2-nitrophenyl beta-D-galactoside 741628 3.2.1.23 45 - transgalactosylation 171352 3.2.1.23 46 - - 171330 3.2.1.23 46 - intracellular enzyme 171308 3.2.1.23 48 - hydrolysis of o-nitrophenyl beta-D-galactopyranoside 654252 3.2.1.23 50 - - 171294, 171295, 171298, 656793, 665789, 666262, 678419, 679960, 682439, 682989, 701854, 702907, 704112, 713787, 715223, 727250, 741727 3.2.1.23 50 - free enzyme 701982 3.2.1.23 50 - hydrolysis of o-nitrophenyl beta-D-galactopyranoside 654292 3.2.1.23 50 - hydrolysis of o-nitrophenyl-beta-D-galactoside, strain L103 665287 3.2.1.23 50 - hydrolysis of p-nitrophenyl-beta-D-galactoside 171305 3.2.1.23 50 - hydrolytic activity 655099 3.2.1.23 50 - immobilized enzyme, substrate 2-nitrophenyl beta-D-galactoside 741628 3.2.1.23 50 - in 4 M KCl and at pH 6.5 727249 3.2.1.23 50 - native enzyme, substrate lactose 741628 3.2.1.23 50 - pH 4.5, 15 min 171325 3.2.1.23 50 - recombinant LacA 677681 3.2.1.23 50 - substrate 2-nitrophenyl-beta-D-galactoside 698407 3.2.1.23 50 55 soluble enzyme 171280 3.2.1.23 50 60 - 701854 3.2.1.23 50 60 recombinant isozyme OsGal1 682457 3.2.1.23 51 - beta-galactosidase I 656940 3.2.1.23 52 - beta-galactosidase II 656940 3.2.1.23 53 - beta-galactosidase III 656940 3.2.1.23 55 - - 171190, 171312, 657206, 679904, 690504, 695663, 701854, 746373 3.2.1.23 55 - by incubating the enzyme with o-nitrophenyl-beta-D-galactoside for 10 min at temperatures ranging from 0°C to 70°C 690504 3.2.1.23 55 - for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside 703072 3.2.1.23 55 - hydrolysis of lactose 171310 3.2.1.23 55 - immobilized enzyme (on Ca-alginate gel beads) 701982 3.2.1.23 55 - substrate lactose 698407 3.2.1.23 55 - temperature optimum for 2-nitrophenyl-beta-D-galactopyranoside 714562 3.2.1.23 55 - two optima, 35°C and 55°C 679236 3.2.1.23 55 60 - 701854 3.2.1.23 55 60 McIlvaine buffer 655169 3.2.1.23 55 60 soluble enzyme 171280 3.2.1.23 56 - in permeabilized cell suspension 713677 3.2.1.23 58 - - 701854 3.2.1.23 58 - extracellular enzyme 171308 3.2.1.23 60 - - 171284, 171303, 171337, 171342, 655054, 656436, 701854, 704131, 704135, 715223, 750529, 750594 3.2.1.23 60 - for hydrolysis of lactose 703072 3.2.1.23 60 - hydrolysis of lactose 654292 3.2.1.23 60 - hydrolysis of lactose in buffer solution 171278 3.2.1.23 60 - immobilized enzyme, substrate lactose 741628 3.2.1.23 60 - in immobilized cell suspension 713677 3.2.1.23 60 65 - 171339 3.2.1.23 65 - - 171316, 171329, 655074, 679560, 695663, 701810, 701854, 701882 3.2.1.23 65 - free enzyme or enzyme immobilized on calcium alginate 664765 3.2.1.23 65 - hydrolysis of lactose in skim milk 171278 3.2.1.23 65 - isozymes BGT I and BGT II 677626 3.2.1.23 65 - strain HM-1 171287 3.2.1.23 65 70 assay at 678474 3.2.1.23 70 - - 699203, 701854, 715223, 743998 3.2.1.23 70 - enzyme immobilized on DEAE-cellulose 664765 3.2.1.23 70 - pH 7.0, maximum activity 699203 3.2.1.23 70 - strain 11-1O 171287 3.2.1.23 75 - - 701854 3.2.1.23 78 - - 171283 3.2.1.23 80 - - 171313, 701854, 701857 3.2.1.23 80 - assay at 722130, 726529, 747376 3.2.1.23 80 - at pH 7.0 171351 3.2.1.23 80 - hydrolysis of lactose 665341 3.2.1.23 80 - hydrolysis of lactose or p-nitrophenyl beta-D-galactopyranoside 654233 3.2.1.23 80 85 - 701854 3.2.1.23 85 - - 171345, 701854 3.2.1.23 85 - hydrolysis of o-nitrophenyl-beta-D-galactoside 665341 3.2.1.23 85 - recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside 725811 3.2.1.23 85 - substrate: 2-nitrophenyl beta-D-galactopyranoside, at pH 6.5, mutant enzymes F441Y and F359Q 722308 3.2.1.23 90 - - 677781, 725579 3.2.1.23 90 - above 680425 3.2.1.23 90 - substrate: 2-nitrophenyl beta-D-galactopyranoside, at pH 6.5, wild-type enzyme 722308 3.2.1.23 93 - - 723162 3.2.1.23 95 - - 679616