3.1.1.41 25 - - 170675 3.1.1.41 25 - assay at 677538, 747164 3.1.1.41 28 - - 651823 3.1.1.41 30 - - 660484 3.1.1.41 35 - - 170672 3.1.1.41 40 - assay at 729073 3.1.1.41 40 50 - 170666, 170667 3.1.1.41 45 - - 649401 3.1.1.41 50 - - 170665, 170669 3.1.1.41 50 - assay at 749261 3.1.1.41 55 - - 170677 3.1.1.41 55 - strain SHS 0133, FERM BP-2755 170673 3.1.1.41 60 70 truncation variants lacking four and ten residues of the N-terminus 749261 3.1.1.41 95 - excessive auto-hydrolysis of the substrate at higher temperatures limits the assay at 95°C 749261 3.1.1.41 100 - - 730907