5.4.99.8 - 5.4.99.8 cloning of full-length gene WsCAS by homology-based PCR method, DNA and amino acid sequence determination and analysis, sequence comparisons, transformation of overexpressing and RNAi constructs into Withania somnifera plants via Agrobacterium strain GV3101 transformation method, quantitative RT-PCR enzyme expression analysis 5.4.99.8 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloning and expression in Escherichia coli strain D5alpha, functional expression of GST-tagged enzyme in Schizosaccharomyces pombe 5.4.99.8 expressed in Escherichia coli and in the yeast mutant GIL77 5.4.99.8 expressed in Saccharomyces cerevisiae mutant GIL77 cells 5.4.99.8 expression in DH5-alpha Escherichia coli competent cells 5.4.99.8 expression in Escherichia coli 5.4.99.8 expression in Sacchaormyces cerevisiae 5.4.99.8 expression in Saccharomyces cerevisiae 5.4.99.8 expression in yeast