4.2.2.5 cloning and construction of mutants in Escherichia coli and expression in Flavobacterium heparinum, the Flavobacterium heparinum strains are mutated by conjugation 650050 4.2.2.5 expressed in Escherichia coli as a His-tagged fusion protein 694963 4.2.2.5 expression in Escherichia coli 747368, 748419, 748523 4.2.2.5 gene cAC, DNA sequence determination and analysis, functional expression without the N-terminal signal peptide in Escherichia coli as His-tagged protein, 2 different expression systems 649575 4.2.2.5 gene cslA, DNA sequence determination and analysis, chromosomal localization, expression in Escherichia coli 649356 4.2.2.5 mutant R292A, expression in Escherichia coli and conjugation to Flavobacterium heparinum to create a mutant strain 650383 4.2.2.5 the gene cslA is expressed in Escherichia coli 663801