4.2.1.B20 cloning of another onoceroid synthase gene that shows high sequence identity with a previously identified alpha-onocerin synthase, functional coexpression with pre-alpha-onocerin synthase in yeast leads to the production of tohogenol and serratenediol 4.2.1.B20 coexpression of onoceroid synthase BmeTC from Bacillus megaterium with Saccharomyces cerevisiae squalene synthase (gene ScERG9) and Alicyclobacillus acidocaldarius squalene-hopene synthase mutant D377C (mutated gene shc) in Escherichia coli strain MEP43 (with deleted the beta-carotene synthesis module) under control of constitutive J23119 promoter, subcloning in Escherichia coli strain DH5alpha 4.2.1.B20 gene BmTC, recombinant coexpression of enzyme BmTC with AaSHC (EC 5.4.99.17) mutant D377C in a squalene epoxidase Erg1-deficient Pichia pastoris strain leading to increased production of (+)-ambrein, recombinant expression of enzyme BmTC mutant D373C 4.2.1.B20 recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)