4.1.99.5 affinity-tagged version expressed in Escherichia coli 4.1.99.5 cloning of gene Synpcc7942_159 from strain PCC7942, overexpression of N-terminally His-tagged protein in Escherichia coli strain BL21(DE3) 4.1.99.5 expressed in Escherichia coli 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli 4.1.99.5 expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 4.1.99.5 gene Npun_R1711, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 4.1.99.5 gene PMT1231, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain BW25113(DE3) DELTA13 4.1.99.5 gene PMT_1231, recombinant expression in Escherichia coli 4.1.99.5 gene Synpcc7942_1593, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha 4.1.99.5 gene Synpcc7942_1593, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)