3.7.1.8 - 3.7.1.8 BphD mutants expressed in a pET-14b vector as an N-terminal His6 fusion protein 3.7.1.8 expression from pBUPH2 in Escherichia coli MV1184 3.7.1.8 expression in Escherichia coli 3.7.1.8 expression in Escherichia coli HB101 3.7.1.8 gene bphD, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 3.7.1.8 gene bphD, expression of wild-type and mutant BphDs 3.7.1.8 heterologously expressed in Escherichia coli BL21 (DE3) 3.7.1.8 into the pET-14b vector, overexpression in Escherichia coli BL21(DE3) cells 3.7.1.8 overexpression in Escherichia coli