3.5.1.115	expressed in Escherichia coli BL21(DE3) cells	
3.5.1.115	gene mca or NCgl0948, quantitative real-time PCR enzyme expression analysis, recombinant wild-type and mutant enzyme expression in Escherichia coli strain BL21(DE3)	
3.5.1.115	the gene is cloned into the pVP56 K [N-terminal His-maltose-binding protein (MBP)] and pFN18 K (N-terminal Halo-tag) expression vectors, expression in Escherichia coli BL21(DE3) cells