3.4.24.21 - 3.4.24.21 astacins genomic organization, phylogenetic analysis 3.4.24.21 astacins genomic organization, phylogenetic analysis, enzyme expression analysis during regeneration, overview 3.4.24.21 astacins genomic organization, phylogenetic analysis, enzyme expression analysis in tissues and during regeneration 3.4.24.21 cDNA reverse-transcribed from midgut-gland mRNA, cloned into pET3a vector and used to transform Escherichia coli BL21(DE3) 3.4.24.21 expressed in Escherichia coli BL21(DE3) cells 3.4.24.21 expression in Hi5 cell 3.4.24.21 gene Ace-mtp-2, DNA and amino acid seuence determination and analysis, recombinant expression in Escherichia coli strains BL21 and Rosetta and Pichia pastoris strain X33 3.4.24.21 gene ASTL, sequence comparisons 3.4.24.21 gene BMP1, sequence comparison with meprin A (EC 3.4.24.18) and meprin B (EC 3.4.24.63) of the astacin enzyme family