3.4.22.B6 - 3.4.22.B6 cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 3.4.22.B6 construction of a intestinal cDNA library, DNA sequence determination and analysis of several genes of the cbl family, analysis of 686 cbl ESTs from USA or UK isolates containing 123 clusters, sequence and antigenic diversity, overview 3.4.22.B6 expressed in Pichia pastoris 3.4.22.B6 expression in insect cells 3.4.22.B6 expression in Pichia pastoris 3.4.22.B6 expression of GST-tagged enzyme in Escherichia coli strain BL21 3.4.22.B6 gene AC-cathB-1, DNA and amino acid sequence determination and analysis, sequence comparisosns and phylogenetic analysis, quantitative real-time RT-PCR expression analysis 3.4.22.B6 gene AC-cathB-2, DNA and amino acid sequence determination and analysis, sequence comparisosns and phylogenetic analysis, quantitative real-time RT-PCR expression analysis 3.4.22.B6 His-tagged version expressed in Pichia pastoris