3.4.22.52	-	
3.4.22.52	a 289 bp fragment for mu-calpain is cloned into the EcoRV site of pBluescript II KS+ vector	
3.4.22.52	cloning of the 21000 Da small subunit and expression in Escherichia coli BL384	
3.4.22.52	complete amino acid sequence of the large subunit is deduced from its cDNA sequence	
3.4.22.52	expressed in Escherichia coli BL21 cells	
3.4.22.52	expressed in Escherichia coli BL21(DE3) cells	
3.4.22.52	expression in Escherichia coli	
3.4.22.52	expression of mutant enzyme C115S in insect cell using a baculovirus system	
3.4.22.52	large catalytic subunit and two of its mutants are expressed in Escherichia coli using the baculovirus Sf9 system, the L-muCANPDELTA3 mutant lacks domain III, mutant L-muCANPDELTA4 lacks the calmodulin-like domain IV. In Sf9 cells co-expression of the inhibitor calpastatin is necessary to prevent autolysis of the L-muCANP subunit, whereas coexpression of the regulatory subunit enhances it. Only very low levels of mRNA of the truncated form L-muCANPDELTA4 are found in bacmid-transfectred Sf9 cells, and it proves impossible to isolate this mutant using the baculovirus expression system	
3.4.22.52	large subunit