3.2.1.153 48 h in Nicotiana benthamiana leaves infiltrated by Agrobacterium tumefaciens C58C1 with entry vector pDONR(TM)Zeo and destination vector pMDC32 3.2.1.153 enzyme expression analysis of isozymes under different water conditions, overview 3.2.1.153 expressed in Pichia pastoris 3.2.1.153 expression in Pichia pastoris 3.2.1.153 fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb 3.2.1.153 gene 1-FEH1, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEH2a, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEH2b, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview 3.2.1.153 gene 1-FEHa, quantitative RT-PCR expression analysis 3.2.1.153 gene FEH-1, real-time PCR quantitative expression analysis