3.2.1.135	all pul genes expressed in Escherichia coli K-12 strains, all pull genes except pulL expressed from plasmid pCHAP1217, pulL expressed from plasmid pCHAP710. Overexpression of gfp-pulL or gfp-pulM in Escherichia coli	
3.2.1.135	Bifidobacterium breve cultured in reinforced clostridial medium, anaeorbic, 37°C. Escherichia coli cultured in Luria Bertani broth, agitated, 37°C. Lactococcus lactis cultured in M17 broth containing 0.5% glucose, 30°C for amplification of vectro pNZ8048 containing only alpha-amylase or pullulanase encoding domains, respectively. Selection of recombinant Escherichia coli with pORI19-apuB on agar containing erythromycin and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside and isopropyl-beta-D-galactopyranoside	
3.2.1.135	development of an an hydrotetracycline-inducible expression system for expression of a neopullulanase in Bacillus subtilis	
3.2.1.135	Escherichia coli DH5alphaF' grown in Luria-Bertani broth, 37°C with plasmid pTZ57R/T, expression in yeast Pichia pastoris with recombinant plasmid pPICZ-BK193 in Yeast Extract Peptone Dextrose	
3.2.1.135	Escherichia coli NovaBlue and Rosetta (DE3) pLysS, cloning and expression host, respectively, with plasmid pET-20b(+), in LB broth, 37°C	
3.2.1.135	expression in Bacillus subtilis	
3.2.1.135	expression in bacteria	
3.2.1.135	expression in Escherichia coli	
3.2.1.135	expression in Escherichia coli BL21 (DE3)	
3.2.1.135	expression in Escherichia coli CodonPlus BL21 (DE3)