3.1.3.80 electroporation of Ax2 cells with the plasmid pJSK166, generated by cloning the full coding sequence into the extrachromosomal expression vector pRHI8 3.1.3.80 expressed in Escherichia coli 3.1.3.80 expressed in Escherichia coli, human recombinant HsMIPP1 protein with a C-terminal myc-poly(His) epitope tag by using the Pichia pastoris expression system