3.1.1.82 - 3.1.1.82 expressed in Escherichia coli as a His-tagged fusion protein 3.1.1.82 PPD type 1 3.1.1.82 PPD type 2 3.1.1.82 recombinant PPD is produced using a glutathione S-transferase (GST) fusion system. The PPD and GST genes are fused to a pGEX-2T vector and expressed in Escherichia coli BL21(DE3)pLysS strain under the control of a T7 promoter as a fusion protein