2.7.6.2 Escherichia coli strain KL21 (DE3)pLysS is transformed with the expression vector coding for a histidine-tagged wild-type or mutant hTPK1 642743 2.7.6.2 expressed in Escherichia coli BL21 cells 722395 2.7.6.2 gene CA1462, expression in Escherichia coli 697004 2.7.6.2 gene tnr3 642757 2.7.6.2 gene TPK1, TPK1 Sanger sequencing analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS 739080 2.7.6.2 isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts 676560 2.7.6.2 overexpression in Escherichia coli 661335 2.7.6.2 TPK1 642755 2.7.6.2 TPK1 gene, located in 7q34-36, genotyping in 964 individuals from 220 families of European ancestry revealing an association between maternal rs228584 genotype and offspring birth weight 695592