2.7.4.16	construction of the thiamine monophosphate kinase (thiL) overexpression plasmid, overexpression from pCLK710	
2.7.4.16	encoded by thiL, thiL is not transcriptionally regulated by thiamine or thiamine diphosphate	
2.7.4.16	expression in Escherichia coli	
2.7.4.16	expression in Escherichia coli BL21	
2.7.4.16	expression in Escherichia coli Rosetta BL21(DE3)R3	
2.7.4.16	gene THI80 or Pcal_0657, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha