2.7.11.7	-	
2.7.11.7	cells overexpressing GFP-GEF have both unphosphorylated and autophosphorylated MHCK A	
2.7.11.7	cloning of wild-type and mutants of the catalytic domain of myosin I heavy chain kinase as His-tagged proteins and expression in Spodoptera frugiperda Sf9 cells via baculovirus infection system	
2.7.11.7	construction of His-tagged or FLAG-tagged N-terminal enzyme peptides by site directed mutagenesis for introduction of start and stop codons, expression in Escherichia coli BL21(DE3)	
2.7.11.7	construction of His-tagged or GFP fusion proteins with the full length enzyme or enzyme fragments, expression in Dictyostelium discoideum cells	
2.7.11.7	DNA and amino acid sequence determination and analysis	
2.7.11.7	DNA sequence determination and analysis	
2.7.11.7	DNA sequence determination and analysis, gene MHCK possesses all of the domains characteristic of members of the protein kinase C family	
2.7.11.7	expressed in Escherichia coli BL21(DE3) cells	
2.7.11.7	expression of FLAG-tagged T825A and T825E enzyme mutants in Dictyostelium discoideum cells, expression of His-tagged wild-type and mutant MHCK A alpha-kinase domains in Escherichia coli strain BL21(DE3)