2.7.1.68	-	
2.7.1.68	a yellow fluorescent (YFP) und GUS fusion PIP5K3 protein is expressed in Escherichia coli cells and transformed in Arabidopsis thaliana to produce trangenic plants. Overexpression and mutant is investigated.	
2.7.1.68	cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6 from hippocampus, expression in COS-7 cells	
2.7.1.68	cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, expression in COS-7 cells	
2.7.1.68	cloning of splice variant phosphatidylinositol 4-phosphate 5-kinase Igamma_v6, expression in COS-7 cells. In humans there is an alternative splice site 78 residues into exon 16c that splices out the rest of the Igamma_v5 insert, and then jumps either 1. to the start of exon 17 to generate human Igamma_v3 (the stop codon is then at the start of human exon 18), or 2. straight to that stop codon in exon 18 to generate human Igamma_v6, cloning of splice variants phosphatidylinositol 4-phosphate 5-kinase Igamma_v3, and Igamma_i2 and expression analysis	
2.7.1.68	coexpression of wild-type HA-tagged isozyme alpha or HA-tagged isozyme alpha mutant D227A with HA-tagged Arf6 or HA-tagged Arf6 mutant N121I in COS-1 cells	
2.7.1.68	EmPIP5K gene, DNA and amino acid sequence determination and analysis, located at position 710-8036 nt on the complimentary strand, structural organization of the PIP5K gene, and phylogenetic analysis and tree	
2.7.1.68	enzyme form PIP5KIalpha	
2.7.1.68	expressed as His6-tagged protein in Escherichia coli BL21(DE3) cells	
2.7.1.68	expressed in Chinese hamster ovary cells