2.7.1.49 - 641661, 738451 2.7.1.49 cDNA clone pBTH1 encoding bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase established in Escherichia coli DH5alpha, expressed in Escherichia coli NI500 and Salmonella typhimurium DM456 by functional complementation 639726 2.7.1.49 coexpression of and functional complementation by His-tagged soluble Thi20p or Thi21p isozymes with a thiamin synthetase in an Escherichia coli strain SN372L-3-9-10 deficient in thiamin biosynthesis 661537 2.7.1.49 Escherichia coli BL21(DE3) (pLysS) transformed with pTRD63 carrying ORF2, single gene thiD/J encodes 2 distinct enzyme activities, HMP kinase and HMP-P kinase, functional complemetation of Escherichia coli NI500, overexpression of ThiD 641661 2.7.1.49 expressed in Escherichia coli 671903 2.7.1.49 expressed in Escherichia coli strain BLR (DE3) 672477 2.7.1.49 expression in Escherichia coli 661316 2.7.1.49 gene annotated ThiD, instead of YjbV, subcloning in Escherichia coli strain DH5alpha, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 662007 2.7.1.49 gene thiD, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) 758702 2.7.1.49 overexpression in Escherichia coli 686824