2.7.1.151 DNA and amino acid sequence determination and analysis, no functional complementation of yeast mutant strains, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 640974 2.7.1.151 DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein 660946 2.7.1.151 Examinations of plants deficient in IPMK suggest roles in pollen germination, root growth, as well axillary shoot branching. 686905 2.7.1.151 expressed in Lycopersicon esculentum cv. PKM 706854 2.7.1.151 expression in bacteria as glutathione S-transferase fusion protein 393221 2.7.1.151 expression in enzyme-deficient yeast cells, stable overexpression in HEK-293 cells, abrogates the in vivo elevation of 1D-myo-inositol 1,4,5,6-tetrakisphosphate from the Salmonella dublin SopB protein 660926 2.7.1.151 expression in HEK293 cells as glutathione S-transferase fusion protein 393220 2.7.1.151 expression in Saccharomyces cerevisiae. A lysine accumulation phenotype of ScIpk2DELTA mutant yeast is rescued by Solanum tuberosum inositol phosphate multikinase 671894 2.7.1.151 Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria. 689562 2.7.1.151 expression of HA-tagged enzyme in human HEK293 and in COS-7 cells 663233