2.7.1.137 - 587104, 640888, 640916, 640936, 640950, 640957, 640959, 640961, 674783 2.7.1.137 C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K 640931 2.7.1.137 CaVPS34 gene under the control of its own promoter is not able to complement the temperature-sensitive growth of Saccharomyces cerevisiae vps34, overexpression of CaVPS34 is sufficient to rescue the temperature-sensitive vps34 phenotype, suggesting a functional conservation in Candida albicans 640934 2.7.1.137 class Ia isozymes, DNA sequence determination and analysis, expression of His-tagged isozymes or P85 and p110 subunits alone in Spodoptera frugiperda Sf9 insect cells utilizing the baculovirus infection system 663296 2.7.1.137 class II phosphoinositide 3-kinase with a C2 domain, cloned from a U937 monocyte cDNA library, expression in mammalian and insect cells 640862 2.7.1.137 class II phosphoinositide 3-kinase, PI 3-kinase C2b, with a C2 domain is cloned from a U937 monocyte cDNA library and expressed in mammalian and insect cells 640862 2.7.1.137 cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin 640880 2.7.1.137 cloning, structural organization, and chromosomal localization of the mouse PI3-kinase p110a gene 640859 2.7.1.137 construction of a tetracycline-inducible expression system by introducing a regulatory plasmid, which constitutively expresses tetracycline-sensitive transactivator, and a response plasmid, containing a tetracycline response element in form of a heptameric repeat of the tetR binding site, under control of a minimal CMV promotor, into Jurkat cells, individual and coexpression of the Myc-tagged enzyme subunits in the Jurkat cell system, determination of expression levels, overview 662576 2.7.1.137 DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged TcVps34 in Escherichia coli strain BL21(DE3) and complementation of an enzyme-deficient Saccharomyces cerevisiae knockout mutant strain 708996