2.5.1.59	-	
2.5.1.59	alpha-subunit encoded by RAM2 and beta-subunit encoded by CDC43 translationally coupled by overlapping the RAM-CDC43 stop-start codons and by locating a ribosome-binding site near the 3' end of RAM2, recombinant enzyme overproduced in Escherichia coli	
2.5.1.59	alpha-subunit encoded by RAM2 cloned to the pFC vector, which has an chloroamphenicol resistance gene and beta-subunit encoded by CAL1 cloned to the pFlag vector, which has an ampicillin resistance gene, these two recombinant enzymes are cotransformed and expressed in Escherichia coli	
2.5.1.59	coexpression of two subunits of enzyme by Sf9 cells infected with recombinant baculovirus	
2.5.1.59	expressed in Escherichia coli	
2.5.1.59	expressed in Escherichia coli BL21 (DE3) and Sf9 insect cells	
2.5.1.59	expressed in Escherichia coli strain BL21(DE3) GGPT/pET23a	
2.5.1.59	expressed in Sf9 insect cells	
2.5.1.59	gene cdc43, encoding the Ggtase-I beta-subunit, DNA and amino acid sequence determination and analysis, recombinant expression of wild-ype and mutant enzymes	
2.5.1.59	identification of the cwp1+ gene, which encodes the alpha-subunit of enzyme, coexpression of cwp1p and cwg2p, beta-subunit, in Escherichia coli