2.4.2.18 - 638678, 638679, 638680 2.4.2.18 amplified fragments containing the full-length trpD gene ligated into the pQE40 vector, transformed into Escherichia coli competent DH5alpha cells. Wild-type and its mutants expressed heterologously in Escherichia coli strain W3110 trpEA2trpEA2, containing the helper plasmid pDM,1 702308 2.4.2.18 expressed in Escherichia coli 735669 2.4.2.18 expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells 759199 2.4.2.18 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells 759160 2.4.2.18 expressed in Escherichia coli M15 cells 760106 2.4.2.18 expression in Escherichia coli 638678 2.4.2.18 His-tagged protein expressed in Escherichia coli 721132 2.4.2.18 wild-type and mutants in pQE40 for expression with N-terminal hexa-His tag in Escherichia coli W3110 trpEA2 (pDM) 693629